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. 1981 Dec 18;214(4527):1353-5.
doi: 10.1126/science.7313694.

Computer averaging of electron micrographs of 40S ribosomal subunits

J Frank, A Verschoor, M Boublik

Computer averaging of electron micrographs of 40S ribosomal subunits

J Frank et al. Science. .

Abstract

An enhanced lateral view of the 40S ribosomal subunit of HeLa cells has been obtained by computer averaging of single particles visualized in the electron microscope. Application of crystallographic criteria to independent averages shows that the reproducibility of the result is comparable to that obtained for thin, stained protein crystals by conventional Fourier filtration methods.

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