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. 1981 Dec 11;9(23):6369-77.
doi: 10.1093/nar/9.23.6369.

RNA synthesis in isolated yeast mitochondria

RNA synthesis in isolated yeast mitochondria

G S Groot et al. Nucleic Acids Res. .

Abstract

Isolated yeast mitochondria incorporate added UTP into RNA. Amongst the products formed are the two rRNAs, 4S RNA and several components presumed to be mRNAs. In omega+ strains (containing an intervening sequence in the 21S rRNA gene) besides mature 21S rRNA a transcript could be detected still containing nucleotide sequences transcribed from this intervening sequence. In omega- strains (not containing this intervening sequence) also a longer form of the 21S rRNA could be observed. These results suggest that isolated yeast mitochondria are capable of carrying out RNA synthesis and processing, including splicing.

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