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. 1981 Sep 1;197(3):767-70.
doi: 10.1042/bj1970767.

Isolation and partial characterization of pancreatic polypeptide-like material in the brain of the blowfly Calliphora vomitoria

H Duve, A Thorpe, R Neville, N R Lazarus

Isolation and partial characterization of pancreatic polypeptide-like material in the brain of the blowfly Calliphora vomitoria

H Duve et al. Biochem J. .

Abstract

Using 10(6) flies (5 kg of heads) a pancreatic polypeptide-like material has been partially purified from the blowfly Calliphora vomitoria. The isolated material was eluted on Sephadex G-50 similarly to bovine pancreatic polypeptide and had an RF on polyacrylamide-gel electrophoresis that was identical with that of the bovine hormone. The material diluted linearly and showed parallelism with bovine standards in a bovine pancreatic polypeptide immunoassay. In specificity controls the immunoreactivity was not abolished by trasylol and no cross-reactivity was discerned in assay for glucagon, proangiotensin and cyclic AMP. These data suggest that the pancreatic polypeptide material in the brain of the blowfly has close structural similarity to the mammalian hormone.

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References

    1. J Biol Chem. 1975 Dec 25;250(24):9369-76 - PubMed
    1. Biochem J. 1976 May 15;156(2):279-88 - PubMed
    1. Brain Res Bull. 1977 Jan-Feb;2(1):61-5 - PubMed
    1. Recent Prog Horm Res. 1976;33:519-70 - PubMed
    1. Regul Pept. 1980 Dec;1(3):187-204 - PubMed

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