[Studies on the mobilities of granulocytes and the chemotactic factor production by human mononuclear cells (author's transl)]

Abstract

Various types of granulocyte mobilities and chemotactic factor production mononuclear cells were studied by the modified agarose plate methods. Three types of granulocyte mobilities, namely random mobility, chemotaxis, and chemokinesis, are easily measured by the agarose plate method. Enhanced granulocyte random mobility by chemotactic factor was observed even when no gradient of chemotactic factor existed. This chemokinetic response decreased in cord blood leukocytes which was known to have the decreased chemotactic response compared to normal adults control. It was speculated that not only chemotaxis but also chemokinesis play an important role in inflammatory reactions in vivo. The degree of disturbance of chemotactic response of cord blood leukocytes which was observed in agarose plate method, was not so apparent as that which was reported by using Boyden chamber method. It seemed that the deformability of granulocytes contributed on the results of chemotaxis in Boyden chamber method. When zymosan activated serum was used as chemoattractant, the chemotactic response of cord blood leukocytes was more impaired than when E. coli derived factor was used. The same results were obrained when chemotactic response of granulocytes from pediatric patients with malignancies were examined. It was concluded that various chemotactic factors should be used as the chemoattractant when the chemotaxis of granulocytes in any disease was examined. One of the chemotactic factors by granulocytes is cell derived chemotactic factor for granulocytes. Production of chemotactic factor for granulocytes by human mononuclear cells was studied in agarose plate method. It was designated as the chemotactic factor for granulocytes, CFG. CFG was produced by human mononuclear cells when they stimulated with LPS or anti beta 2 microglobulin. CFG producing cells were glass adherent and carbonyl iron phagocyting cells. So it was suggested that CFG producing cells were monocytes. This CFG could not attract monocytes, though zymosan activated serum could attract both monocytes and granulocytes. Moreover, anti human C5 serum could not abrogate the chemotactic activity of CFG. It seemed that CFG was different from complement derived chemotactic factor, C5a. Furthermore, specific antiserum against human C3 or IgG could not also abrogate the chemotactic activity of CFG.