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. 1981;47(3):333-8.
doi: 10.1016/0022-1759(81)90289-1.

A micromethod for the induction and assay of specific in vitro antibody responses by human lymphocytes

E D Zanders, C M Smith, R E Callard

A micromethod for the induction and assay of specific in vitro antibody responses by human lymphocytes

E D Zanders et al. J Immunol Methods. 1981.

Abstract

Human lymphocytes obtained from peripheral blood or spleen were cultured with influenza virus in a microculture system to produce strain specific antibody which was detected by the enzyme linked immunosorbent assay (ELISA). Nanogram amounts of specific antibody could be detected in cultures of 4 x 10(5) cells.

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