Structure and function of the junctional complement of spontaneous and transplanted murine mammary carcinomas

Abstract

In this study the morphology of intercellular junctions in a murine mammary adenocarcinoma and in a solid carcinoma which resulted from continuous transplantations of this spontaneous tumor are described employing the techniques of ultrathin-sectioning after conventional fixation and tannic acid fixation and freeze-fracturing. The acini of the original adenocarcinoma are separated from the intercellular spaces by tight junctions which form a narrow belt-like zonula around the adluminal parts of all epithelial cells. The apical-to-basal width of the tight junctions varies from 0.4 to 0.8 micrometer. Desmosomes and gap junctions are located close to the zonulae occludentes. The size of gap junctions ranges from small spots 0.2 micrometer in diameter to large areas approximately 1.5 micrometer in diameter. In the solid carcinoma these cellular junctions appear without acinar organization randomly between the cells. In addition, special contact zones can be observed. The contact zones seem to be the precursors of a formation plaque for gap or tight junction formation. The sizes of the gap junctions show a wide range of variation, from as small as 5 particles to as large as 0.5 micrometer in diameter. Tight junctions do not form continuous belt-like zonulae indicating that they have lost their significance as a permeability barrier. They are interpreted as mechanical links. In addition to isolated gap junctions and tight junctions there exist gap junctions adjacent to tight junction fibrils suggesting a probable biogenetic relationship of these two structures.