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Comparative Study
. 1981 Dec 21;9(24):6787-94.
doi: 10.1093/nar/9.24.6787.

Altered mobility of polydeoxyribonucleotides in high resolution polyacrylamide gels due to removal of terminal phosphates

D P Tapper, D A Clayton
Comparative Study

Altered mobility of polydeoxyribonucleotides in high resolution polyacrylamide gels due to removal of terminal phosphates

D P Tapper et al. Nucleic Acids Res. .

Abstract

We have compared the electrophoretic mobility of a series of polynucleotides differing solely by the presence or absence of a terminal phosphate. As expected, the effect of removal of a single terminal phosphoryl residue on electrophoretic mobility is dependent on the size of the polynucleotide and therefore is not constant. Removal of a phosphoryl residue from polynucleotides shorter than 30 nucleotides reduces the mobility the equivalent of one nucleotide. Between 30 and 50 nucleotides the reduction in mobility is approximately one-half a nucleotide, while above 50 nucleotides in size the effect of phosphate removal approaches zero.

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References

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