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. 1981 Dec 1;199(3):789-93.
doi: 10.1042/bj1990789.

Identification of fatty acid synthetase messenger RNA on free polyribosomes isolated from lactating rabbit mammary gland

M Antoniou, R Craig, R Dils

Identification of fatty acid synthetase messenger RNA on free polyribosomes isolated from lactating rabbit mammary gland

M Antoniou et al. Biochem J. .

Abstract

The synthesis of fatty acid synthetase on free polyribosomes from lactating rabbit mammary gland was demonstrated by using polyribosomes run-off techniques and immunochemical identification of products with synthetase antiserum. Several reproducible and discrete immunoprecipitable polypeptides were observed which were within the molecular-weight range of the synthetase subunit (235 000--252 000), as well as several of lower molecular weight.

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