Uptake of amino acids and glucose by BHK 21 clone 13 suspension cells during cell growth

Abstract

The uptake of amino acids and glucose by BHK 21 clone 13 suspension cells grown in Modified Eagles Medium was monitored. The Growth Yields for sixteen amino acids and glucose were determined. Four "non essential" amino acids were utilised. Glutamine, glutamate, leucine and asparagine were used in greatest quantity by the cells which also utilised virtually all the cystine in the medium. The implications of these results are discussed.