Separation of granulocytes from peripheral blood in a single step using discontinuous density gradients of Ficoll-Urografin. A comparative study with separation by dextran

Abstract

A single-step method for separating granulocytes from peripheral blood using two different gradients of Ficoll-Urografin (F-U) density 1.075 and 1.097 g/ml) is described. The morphological, cytochemical and ultrastructural properties of neutrophils isolated by this method were compared to those isolated by dextran-enhanced sedimentation and neutrophils from the peripheral blood. These studies indicated fewer alterations in neutrophils separated by F-U centrifugation than in neutrophils concentrated by dextran. Finally, the granulocyte layer recovered by F-U fractionation was 98% pure (dextran 71%) and yielded 64% (dextran 79%) of total granulocytes.