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. 1980 Feb 1;207(4430):525-6.
doi: 10.1126/science.7352259.

Human fibroblast interferon: amino acid analysis and amino terminal amino acid sequence

E Knight Jr, M W Hunkapiller, B D Korant, R W Hardy, L E Hood

Human fibroblast interferon: amino acid analysis and amino terminal amino acid sequence

E Knight Jr et al. Science. .

Abstract

The purification of human fibroblast interferon has been simplified to a two-step procedure consisting of affinity chromatography on Blue Sepharose and sodium dodecyl sulfate polyacrlamide gel electrophoresis. A preliminary amino acid composition and the sequence of the 13 amino-terminal residues of homogeneous interferon prepared by this method is reported.

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