Serotonergic intraventricular axons in the habenular region. Phagocytosis after induced degeneration

Abstract

Both intracerebroventricular injection of 5,7-dihydroxytryptamine and electrolytical midbrain-raphe lesions in rats induce degeneration of supraependymal axons (SEAs) normally occurring in large numbers upon the ependyma of the medial habenular nucleus and habenular commissure. It is concluded that the intraventricular axon plexus in the epithalamic region is comprised of serotonergic (5-HT) fibers originating in the dorsal and/or median raphe nuclei. Besides the elimination of SEAs, conspicious features were a marked reduction in the number of cilia, degenerative signs in the habenular ependyma, and the emergence of large numbers of supraependymal macrophages, being most probably involved in phagocytosis of the axonal debris. It is suggested that the nucleus habenulae medialis is influenced serotonergically by the midbrain raphe via (1) a direct projection upon its neurons and (2) an indirect projection by way of the intraventricular axon plexus. The origin of intraventricular macrophages is discussed in relation to recent data in the literature.