Methods for the identification of human B lymphocytes

Abstract

Complement-receptor lymphocytes and monocytes were identified by a rosette method using trypsin treated sheep erythrocytes (Et), sensitized with affinity column purified IgM (19S) antibodies against sheep erythrocytes (E) and mouse complement deficient in C5. Fc receptor mononuclear cells were identified by a rosette method using Et and IgG (7S) antibodies against E. Identification of the cell-type rosetting was facilitated by myeloperoxidase staining of dry mounted rosetted preparations. Comparison of lymphocytes with receptors for activated mouse complement and lymphocytes with stable surface immunoglobulin detected by immunofluorescent assay, strongly suggests that these cells constitute the same population in the peripheral blood of healthy humans.