Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1980 Feb;124(2):766-8.

A hybridoma from an autoimmune NZB/NZW mouse producing monoclonal antibody to ribosomal-RNA

D Eilat, R Asofsky, R Laskov
  • PMID: 7356711

A hybridoma from an autoimmune NZB/NZW mouse producing monoclonal antibody to ribosomal-RNA

D Eilat et al. J Immunol. 1980 Feb.

Abstract

Spleen cells from an unimmunized autoimmune NZB/NZW F1 female mouse were fused to the BALB/c MPC-11 drug-resistant clone 45.6 TG 1.7. One hybrid clone (D4) produced IgG-3 immunoglobulin that bound ribosomal RNA. A high concentration of this antibody was produced in the ascitic fluid of NZB/NZW male mice. DNA, tRNA, and synthetic single- and double-stranded polynucleotides could not bind significantly to the antibody. A Scatchard analysis showed that the rRNA-binding immunoglobulin is monoclonal and has a high affinity (10(9) liter/mole) for the RNA antigen.

PubMed Disclaimer

LinkOut - more resources