doi: 10.1016/s0021-9673(00)87882-6.
Determination of clonidine in human plasma by glass capillary gas chromatography with electron-capture detection
- PMID: 7358813
- DOI: 10.1016/s0021-9673(00)87882-6
Item in Clipboard
Determination of clonidine in human plasma by glass capillary gas chromatography with electron-capture detection
J Chromatogr.
.
Abstract
A gas chromatography assay for clonidine in human plasma has been developed. The buffered serum is extracted on silica columns, alkylated with pentafluorobenzyl bromide, cleaned up-by extractions and analysed by glass capillary gas chromatography with falling-glass-needle injection and electron-capture detection. A gas chromatographic-mass spectrometric analysis of the derivatives formed and an investigation of the structure dependence of the electron-capture response are presented.