doi: 10.1111/j.1365-2818.1977.tb00035.x.
Polymeric cryoprotectants in the preservation of biological ultrastructure. II. Physiological effects
- PMID: 73592
- DOI: 10.1111/j.1365-2818.1977.tb00035.x
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Polymeric cryoprotectants in the preservation of biological ultrastructure. II. Physiological effects
J Microsc.
1977 Aug.
Abstract
A study has been made of the physiological effects of three non-penetrating polymeric cryoprotective agents on sixteen different plant and animal cells and tissues. The cryoprotectants, when used at concentrations at which they are effective in preventing ice-crystal formation, generally have a lower toxicity to cells and tissue than similar concentrations of glycerol. The relatively low toxicity of these substances suggests that they would be more suitable as cryoprotectants for morphological and analytical studies than the commonly used low molecular weight compounds.
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