Changes of 32P-incorporation in vitro into phospholipids of blood platelets after whole-body irradiation of rabbits. I. Comparison of 32P-incorporation with some platelet functional tests during early development of postirradiation thrombocytopenia

Abstract

In the period of postirradiation bone-marrow activity depression, changes of the 32P incorporation into phospholipids of blood platelets in rabbits after whole-body exposure to 103.2 mC/kg were noted in vitro. Increased incorporation into phosphatidylcholine, phosphatidylethanolamine and sphingomyeline on the 11th day and into phosphatidylserine on the 8th and 11th day were recorded. On the 5th, 8th and 11th days the pattern of incorporation is changed. During this period a pronounced decrease of 32P incorporation into phosphatidylinositols of the blood platelets was noted, no changes in the representation of the phospholipids of interest (PC, PE, SM, PS, PI) were observed and no alterations of the blood platelet adhesiveness were found. The PF3-A and PF3-F test did not reveal any significant changes of the role of PF 3 in the blood clotting process.