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. 1980 Mar;19(3):741-51.
doi: 10.1016/s0092-8674(80)80050-x.

Processing of intervening sequences: a new yeast mutant which fails to excise intervening sequences from precursor tRNAs

A K Hopper, L D Schultz, R A Shapiro

Processing of intervening sequences: a new yeast mutant which fails to excise intervening sequences from precursor tRNAs

A K Hopper et al. Cell. 1980 Mar.

Abstract

By using conditional loss of suppression an an assay, we have been successful in screening for a yeast mutant which is defective in tRNA processing. The los1-1 mutation causes an accumulation of a subset of precursor tRNAs at the nonpermissive temperature. These pre-tRNAs are like those which accumulate in the yeast mutant ts 136 (rna1) in that they have transcribed intervening sequences. The mutations at los1-1 and rna1 complement and segregate independently of each other. The los1-1 mutation affects the expression of all 8 tyrosine-inserting suppressor loci, but does not seem to affect rRNA or mRNA synthesis.

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