[Properties of alpha-chymotrypsin, covalently incorporated into polyacrylamide microgranules]
[Article in
Russian]
- PMID: 7378483
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[Properties of alpha-chymotrypsin, covalently incorporated into polyacrylamide microgranules]
[Article in
Russian]
Biokhimiia.
1980 Mar.
Abstract
Polyacrylamide beads (microgranules) containing covalently bound alpha-chymotrypsin were obtained. An average diameter of the microgranules is 100--150 micrometers. Prior to the bead polymerization alpha-chymotrypsin was modified by acryloyl chloride. The efficiency of the microgranulated alpha-chymotrypsin action depends on the kinetic properties of the substrates used. The pH optimum for the microgranulated enzyme activity towards N-acetyl-L-tyrosine ethyl ester is shifted by 1,5 units to the alkaline zone. The immobilized alpha-chymotrypsin is completely stable for 100 hrs at 50 degrees.
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