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. 1980 Jun 5;285(5764):367-73.
doi: 10.1038/285367a0.

Specific in vitro initiation of transcription on conalbumin and ovalbumin genes and comparison with adenovirus-2 early and late genes

B Wasylyk, C Kédinger, J Corden, O Brison, P Chambon

Specific in vitro initiation of transcription on conalbumin and ovalbumin genes and comparison with adenovirus-2 early and late genes

B Wasylyk et al. Nature. .

Abstract

Specific initiation of transcription of cloned conalbumin and ovalbumin genes has been obtained in a cell-free system in the presence of purified calf thymus RNA polymerase B. Comparison with the adenovirus-2 early (E1A) and major late genes reveals marked variations in transcriptional efficiencies. Specific fragmentation of the conalbumin gene DNA indicates that a short DNA segment 5' to the initiation site is involved in specific transcription.

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