doi: 10.1016/0090-6980(80)90156-2.
Preparation and characterization of hydroperoxy-eicosatetraenoic acids (HPETEs)
- PMID: 7384539
- DOI: 10.1016/0090-6980(80)90156-2
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Preparation and characterization of hydroperoxy-eicosatetraenoic acids (HPETEs)
Prostaglandins.
1980 Jan.
Abstract
5-, 8-, 9-, 11-, 12- AND 15 hydroperoxy-eicosatetraenoic acids (HPETEs) were generated from arachidonic acid by a reaction with H2O2 and Cu++ ions. They were purified by high performance liquid chromatography, either on a silica gel (mu Porasil) column or on a reversed phase (mu Bondapak C18) column. The yield of 5-HPETE was considerably greater when the mu Bondapak C18 column was used. The HPETEs were characterized and assayed by their ability to oxidize triphenylphosphine: triphenylphosphine oxide formation was monitored by gas chromatography. When stored in methylene chloride at -20 degrees C, the HPETES were stable for several months.
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