Use of DEAE-cellulose filters in the S1 nuclease method for bacterial deoxyribonucleic acid hybridization
- PMID: 7387054
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Use of DEAE-cellulose filters in the S1 nuclease method for bacterial deoxyribonucleic acid hybridization
Ann Microbiol (Paris).
1980 Mar-Apr.
Abstract
Polynucleotide sequence relatedness can be studied with the S1 nuclease method by a fast and accurate procedure using DEAE-cellulose filters (DE81 Whatman). After S1 treatment of DNA-DNA hybrid molecules formed in 0.42 M saline solution, free nucleotides (digestion products) but not DNA are eluted from DE81 filters in appropriate salt concentration such as 5% Na2HPO4.12 H2O. The relative binding ratio and the thermal stability of heteroduplexes are determined for 7 strains of Enterobacteriaceae. The conclusions resulting from S1 nuclease and DE81 filters assay are similar to those obtained with S1 nuclease and trichloracetic acid precipitation.
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