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. 1980;64(2):155-66.
doi: 10.1007/BF01318019.

The protein pattern of PMF virus, a type-D retrovirus from malignant permanent human cell lines

W Uckert, V Wunderlich, E Bender, G Sydow, D Bierwolf

The protein pattern of PMF virus, a type-D retrovirus from malignant permanent human cell lines

W Uckert et al. Arch Virol. 1980.

Abstract

The protein pattern of a type-D retrovirus (PMFV) isolated from and propagated in human cell lines has been investigated using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Staining with Coomassie blue and labeling with 14C-leucine/14C-lysine revealed five viral polypeptides with molecular weights of 10,000, 12,000, 15,000, 25,000, and 68,000. The 68,000 D-protein was shown to be a glycoprotein by incorporation of 3H-glucosamine and the 15,000 D-protein was identified as a phosphoprotein. By comparing PMFV with the closely related Mason-Pfizer monkey virus (MPMV) in co-electrophoresis experiments no clear difference was detected in viral 14C-leucine/14C-lysine profiles. The viruses differ, however, with respect to their glycoprotein patterns. A glycoprotein corresponding to the gp20 of MPMV has not been detected in PMFV irrespective of the cell line used for propagation of viruses.

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