The regulation of carbohydrate metabolism in animal cells: isolation of a glycolytic variant of Chinese hamster ovary cells

Abstract

A variant of Chinese hamster ovary cells is described which is deficient in two glycolytic enzymes: glucosephosphate isomerase and phosphoglycerate kinase. The variant grows readily on glucose, but in contrast to the parental cell it does not excrete lactic acid. The impaired ability of the variant to perform aerobic glycolysis both in vivo and in vitro is correlated with the deficiency in both glycolytic enzymes.