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. 1980 May 30;607(3):527-9.
doi: 10.1016/0005-2787(80)90163-x.

Isolation of the newly synthesized DNA of HeLa cells containing beta-2'-deoxy-6-thioguanylate on an organomercurial agarose column

S Yoshida, T Morita, M Saneyoshi

Isolation of the newly synthesized DNA of HeLa cells containing beta-2'-deoxy-6-thioguanylate on an organomercurial agarose column

S Yoshida et al. Biochim Biophys Acta. .

Abstract

It has been shown that beta-2'-deoxy-6-thioguanosine is incorporated into mammalian DNA via its triphosphate form. The newly synthesized DNA of HeLa cells containing thioguanosine, adsorbed specifically on an organomercurial agarose column and was eluted with 2-mercaptoethanol. By recycling this affinity chromatography, the newly synthesized DNA strands were separated successfully from non-adsorbable parental strands. This system may provide a new tool for the study of DNA replication.

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