doi: 10.1007/BF00332528.
Cold-sensitivity of a double mutant strain combining two ribosomal mutations in the ascomycete Podospora anserina
- PMID: 739974
- DOI: 10.1007/BF00332528
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Cold-sensitivity of a double mutant strain combining two ribosomal mutations in the ascomycete Podospora anserina
Mol Gen Genet.
.
Abstract
A double mutant strain combining two ribosomal mutations conferring resistance to cycloheximide exhibits a cold-sensitive phenotype. At low temperature the biosynthesis of the 60S subunit is impaired. Genetic analysis of cold-resistant revertants have shown that this double mutant strain can be used efficiently to isolate new ribosomal mutations.
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