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. 1978 Oct 16;4(3):171-5.
doi: 10.1007/BF00777519.

A new high sensitive analytical micro-scale procedure for chromosomal proteins

H Hesslinger, K Pollow

A new high sensitive analytical micro-scale procedure for chromosomal proteins

H Hesslinger et al. Mol Biol Rep. .

Abstract

Utilizing male rat liver cells we describe a method for isolating and fractionating chromosomal proteins. About 99% of chromosomal proteins was dissociated using a three step dissociation procedure. DNA was removed by sedimentation and the histone fractions were separated from the non-histone chromosomal proteins by Bio Rex 70 chromatography. The non-histone chromosomal proteins were fractionated by micro-gradient electrophoresis on SDS-polyacrylamide gels, which proved to be superior to the electrophoretic procedures currently in use. The histones were further separated on polyacrylamide-SDS slab gels using a micro-two-dimensional electrophoretic system. The high resolution of these fractionation procedures greatly enhances the possibility of observing small changes in proteins which may play a role in gene regulation.

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