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Comparative Study
. 1980 Jun;33(6):562-5.
doi: 10.1136/jcp.33.6.562.

An evaluation of the British Pharmacopoeial assay of heparin: a comparison with other methods

Comparative Study

An evaluation of the British Pharmacopoeial assay of heparin: a comparison with other methods

G A Shah et al. J Clin Pathol. 1980 Jun.

Abstract

The potencies of six commercially manufactured heparins have been measured by the British Pharmacopoeial (BP) assay and activated partial thromboplastin time (APTT), protamine sulphate, and anti-Xa assays. The APTT/BP potency ratios were found to vary with the preparation but this was not dependent on the tissue source of heparin. For mucosal heparins, the anti-Xa/BP potency ratios were close to unity, but for heparin of lung origin the anti-Xa potency was approximately one-quarter of the BP potency. Four heparin fractions prepared by column gel chromatography of a commercial heparin were similarly examined by all four assays, and there was a wide divergence between the BP potency estimates and those obtained with the other methods. The degree of divergence was found to depend on the molecular size of the fraction.

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References

    1. Can J Physiol Pharmacol. 1967 Sep;45(5):787-94 - PubMed
    1. J Clin Pharmacol New Drugs. 1972 Oct;12(10):413-6 - PubMed
    1. Clin Pharmacol Ther. 1973 Mar-Apr;14(2):287-90 - PubMed
    1. Thromb Diath Haemorrh. 1973 Dec 31;30(3):471-9 - PubMed
    1. Curr Ther Res Clin Exp. 1975 Jul;18(1 Pt1):91-103 - PubMed

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