doi: 10.1126/science.7403880.
Human cutaneous lieshmania in a mouse macrophage line: propagation and isolation of intracellular parasites
- PMID: 7403880
- DOI: 10.1126/science.7403880
Item in Clipboard
Human cutaneous lieshmania in a mouse macrophage line: propagation and isolation of intracellular parasites
Science.
.
Abstract
A mouse macrophage line, J774G8, supports continuous and prolific intracellular growth of Leishmania mexicana amazonensis, the etiological agent of a South American cutaneous leishmaniasis. The intracellular parasites from these infected cultures can be isolated with high recovery rate and purity by simple Percoll gradient centrifugation.
Similar articles
-
Quantification of Intracellular Growth Inside Macrophages is a Fast and Reliable Method for Assessing the Virulence of Leishmania Parasites.J Vis Exp. 2018 Mar 16;(133):57486. doi: 10.3791/57486. J Vis Exp. 2018. PMID: 29608175 Free PMC article.
-
Resistance of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis to nitric oxide correlates with disease severity in Tegumentary Leishmaniasis.BMC Infect Dis. 2007 Feb 22;7:7. doi: 10.1186/1471-2334-7-7. BMC Infect Dis. 2007. PMID: 17316450 Free PMC article.
-
Distinct Macrophage Fates after in vitro Infection with Different Species of Leishmania: Induction of Apoptosis by Leishmania (Leishmania) amazonensis, but Not by Leishmania (Viannia) guyanensis.PLoS One. 2015 Oct 29;10(10):e0141196. doi: 10.1371/journal.pone.0141196. eCollection 2015. PLoS One. 2015. PMID: 26513474 Free PMC article.
-
Leishmania parasites and their ploys to disrupt macrophage activation.Curr Opin Hematol. 2000 Jan;7(1):26-31. doi: 10.1097/00062752-200001000-00006. Curr Opin Hematol. 2000. PMID: 10608501 Review.
-
Intracellular growth and pathogenesis of Leishmania parasites.Essays Biochem. 2011;51:81-95. doi: 10.1042/bse0510081. Essays Biochem. 2011. PMID: 22023443 Review.
Cited by
-
Destruction of Leishmania mexicana amazonensis amastigotes within macrophages in culture by phenazine methosulfate and other electron carriers.J Exp Med. 1982 Feb 1;155(2):415-31. doi: 10.1084/jem.155.2.415. J Exp Med. 1982. PMID: 7057140 Free PMC article.
-
Antileishmanial activity of sodium stibogluconate fractions.Antimicrob Agents Chemother. 1993 Sep;37(9):1842-6. doi: 10.1128/AAC.37.9.1842. Antimicrob Agents Chemother. 1993. PMID: 8239593 Free PMC article.
-
Monoclonal antibody affinity purification of a Leishmania membrane glycoprotein and its inhibition of leishmania-macrophage binding.Proc Natl Acad Sci U S A. 1986 Jan;83(1):100-4. doi: 10.1073/pnas.83.1.100. Proc Natl Acad Sci U S A. 1986. PMID: 3079902 Free PMC article.
-
Immunogenicity and Protective Efficacy of T-Cell Epitopes Derived From Potential Th1 Stimulatory Proteins of Leishmania (Leishmania) donovani.Front Immunol. 2019 Feb 28;10:288. doi: 10.3389/fimmu.2019.00288. eCollection 2019. Front Immunol. 2019. PMID: 30873164 Free PMC article.
-
The Leishmania receptor for macrophages is a lipid-containing glycoconjugate.EMBO J. 1985 Feb;4(2):329-36. doi: 10.1002/j.1460-2075.1985.tb03633.x. EMBO J. 1985. PMID: 4018028 Free PMC article.
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
