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. 1980 Sep 12;209(4462):1240-42.
doi: 10.1126/science.7403880.

Human cutaneous lieshmania in a mouse macrophage line: propagation and isolation of intracellular parasites

K P Chang

Human cutaneous lieshmania in a mouse macrophage line: propagation and isolation of intracellular parasites

K P Chang. Science. .

Abstract

A mouse macrophage line, J774G8, supports continuous and prolific intracellular growth of Leishmania mexicana amazonensis, the etiological agent of a South American cutaneous leishmaniasis. The intracellular parasites from these infected cultures can be isolated with high recovery rate and purity by simple Percoll gradient centrifugation.

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