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. 1980 Apr 15;188(1):137-40.
doi: 10.1042/bj1880137.

The effect of fluoride on the state of aggregation of adenylate cyclase in rat liver plasma membranes

B R Martin, J M Stein, E L Kennedy, C A Doberska

The effect of fluoride on the state of aggregation of adenylate cyclase in rat liver plasma membranes

B R Martin et al. Biochem J. .

Abstract

Irradiation inactivation was used to monitor changes in the state of adenylate cyclase in rat liver plasma membranes in the presence of F-.F- caused a decrease in the target size from 328000 to 237000 at 0 degrees C and from 329000 to 219000 at 30 degrees C. Adenylate cyclase was activated by F- at both 0 degrees C and 30 degrees C. The effect of F- was biphasic, activating up to a concentration of 10mM and inhibiting at higher concentrations. If adenylate cyclase weas maximally activated with glucagon and p[NH]ppG ([beta gamma-imido]GTP) all concentrations of F- were inhibitory. The implications of the results with respect to the mechanism of activation of adenylate cyclase are discussed.

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