doi: 10.1021/bi00557a023.
Multistep mechanism of codon recognition by transfer ribonucleic acid
- PMID: 7407070
- DOI: 10.1021/bi00557a023
Item in Clipboard
Multistep mechanism of codon recognition by transfer ribonucleic acid
Biochemistry.
.
Abstract
The mechanism of codon recognition by tRNA is investigated in the system tRNAPhe + UUC by temperature-jump measurements using the Wye base fluorescence as a label. In 0.4 M Na+ and 5 mM Mg2+ a two-step reaction is observed and described quantitatively; UUC is shown to bind preferentially to one of two conformations on the anticodon loop. In 0.1 m Na+ and 10 mM Mg2+ an additional relaxation effect is observed, which indicates a codon-induced conformation change leading to an association of tRNA molecules. The codon-induced tRNA association is demonstrated independently by equilibrium sedimentation. The present results suggest a more active role of tRNA during translation than anticipated.
Similar articles
-
Mechanism of codon recognition by transfer RNA studied with oligonucleotides larger than triplets.Nucleic Acids Res. 1985 May 24;13(10):3667-83. doi: 10.1093/nar/13.10.3667. Nucleic Acids Res. 1985. PMID: 4011439 Free PMC article.
-
Codon-induced association of the isolated anticodon loop of tRNAPhe.Biochemistry. 1986 Oct 21;25(21):6372-8. doi: 10.1021/bi00369a005. Biochemistry. 1986. PMID: 3539190
-
Mechanism of codon recognition by transfer RNA and codon-induced tRNA association.J Mol Biol. 1984 Apr 25;174(4):587-604. doi: 10.1016/0022-2836(84)90085-8. J Mol Biol. 1984. PMID: 6563090
-
On codon- anticodon interactions.Mol Biol Biochem Biophys. 1980;32:347-67. doi: 10.1007/978-3-642-81503-4_27. Mol Biol Biochem Biophys. 1980. PMID: 7003350 Review. No abstract available.
-
[The basis of high-fidelity translation].Mol Biol (Mosk). 1984 Sep-Oct;18(5):1163-80. Mol Biol (Mosk). 1984. PMID: 6390170 Review. Russian.
Cited by
-
GTPase center of elongation factor Tu is activated by occupation of the second tRNA binding site.Proc Natl Acad Sci U S A. 1986 Jul;83(13):4617-21. doi: 10.1073/pnas.83.13.4617. Proc Natl Acad Sci U S A. 1986. PMID: 3014498 Free PMC article.
-
The solution structure of a RNA pentadecamer comprising the anticodon loop and stem of yeast tRNAPhe. A 500 MHz 1H-n.m.r. study.Biochem J. 1984 Aug 1;221(3):737-51. doi: 10.1042/bj2210737. Biochem J. 1984. PMID: 6089745 Free PMC article.
-
Mechanism of codon recognition by transfer RNA studied with oligonucleotides larger than triplets.Nucleic Acids Res. 1985 May 24;13(10):3667-83. doi: 10.1093/nar/13.10.3667. Nucleic Acids Res. 1985. PMID: 4011439 Free PMC article.
-
Role of Wybutosine and Mg2+ Ions in Modulating the Structure and Function of tRNAPhe: A Molecular Dynamics Study.ACS Omega. 2019 Dec 2;4(25):21327-21339. doi: 10.1021/acsomega.9b02238. eCollection 2019 Dec 17. ACS Omega. 2019. PMID: 31867527 Free PMC article.
-
Effect of ribosome binding and translocation on the anticodon of tRNAPhe as studied by wybutine fluorescence.Nucleic Acids Res. 1982 Apr 24;10(8):2651-63. doi: 10.1093/nar/10.8.2651. Nucleic Acids Res. 1982. PMID: 7043399 Free PMC article.