Binding of non-mammalian immunoglobulins to staphylococcal protein A

Abstract

Immunoglobulins M of the chicken (about 3% of serum IgM), clawed toad (12%) and carp (13%) bind to the protein A-Sepharose. A monomeric immunoglobulin with four domain heavy chains from the chicken serum is also bound (30% of bound immunoglobulins). The protein A-Sepharose can be utilized for isolation of IgM of the clawed toad and carp directly from the serum.