Development of a specific antispermine antibody
- PMID: 7423025
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Development of a specific antispermine antibody
Res Commun Chem Pathol Pharmacol.
1980 Sep.
Abstract
Highly specific antibody to spermine was produced in the rabbits by immunization with a spermine-thyroglobulin conjugate of high molar ratio (740:1). Cross-reactivity with spermidine was 1.4-2.2 percent and negligible with putrescine and other compounds of similar structure. The average affinity constant of five pools of antispermine rabbit antisera was Ka = 1.8 +/- 0.2 X 10(8) liter/mole.
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