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. 1980 Jul;94(3):376-80.
doi: 10.1530/acta.0.0940376.

Effect of receptor occupancy on [3H]dexamethasone binding to circulating leukocytes

T Murakami, D D Brandon, D L Loriaux, M B Lipsett

Effect of receptor occupancy on [3H]dexamethasone binding to circulating leukocytes

T Murakami et al. Acta Endocrinol (Copenh). 1980 Jul.

Abstract

To determine the effect of steroid occupancy of the glucocorticoid receptor on the measurement of total receptor concentration using Scatchard plot of [3H]dexamethasone (Dex) binding to circulating leukocytes, leukocytes were incubated with 10(-7)M cortisol or 10(-9)M Dex prior to [3H]Dex binding study. Total binding capacity calculated from Scatchard plot analysis of [3H]Dex binding was not significantly reduced after pre-incubation with cortisol. However, total binding capacity was significantly lower after pre-incubation with Dex. These data suggest that total glucocorticoid receptor concentration can be measured from a 3 h incubation of leukocytes obtained from untreated patients.

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