[G2 period and entrance into the resting state at different stages of mouse ascitic hepatoma 22A development]

Abstract

In the course of AH22A development the size of G2 subpopulations (G2 period cell of the mitotic cycle) changes relatively little for the most part, slightly decreasing in the terminal stage. Five days before the terminal stage of AH22A the cell subpopulation was found to be in the reversible resting R2 stage (the population egressed from the mitotic cycle in G2 period). The size of the population increased with tumor growth. In the terminal stage, however, its size was insignificant (47%). Therefore, it is not possible to prove that cells accumulate in considerable amounts in the reversible resting R2 stage. This circumstance and a decrease in the size of G2 subpopulation and cell population in advanced tumors during the reversible resting R2 stage evidence that the latter one is of a relatively short duration in the course of cell egress from G2 period of the mitotic cycle in the irreversible resting stage.