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. 1980;36(3-4):285-91.
doi: 10.1016/0022-1759(80)90133-7.

Isolation of human NK cells by density gradient centrifugation

T Timonen, E Saksela

Isolation of human NK cells by density gradient centrifugation

T Timonen et al. J Immunol Methods. 1980.

Abstract

Sedimentation characteristics of human NK cells in discontinuous Percoll density gradients were studied. NK activity against the leukaemic cell line K-562 peaked in a single low density fraction mainly consisting of large granular lymphocytes previously shown to be the principal NK cells in human peripheral blood. Percoll density gradient centrifugation provides a useful tool for analysis of human NK cells and their relationship to other blood mononuclear cells.

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