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. 1978 Dec 1;175(3):793-800.
doi: 10.1042/bj1750793.

The exocellular DD-carboxypeptidase-endopeptidase from Streptomyces albus G. Purification and chemical properties

C Duez, J M Frère, F Geurts, J M Ghuysen, L Dierickx, L Delcambe

The exocellular DD-carboxypeptidase-endopeptidase from Streptomyces albus G. Purification and chemical properties

C Duez et al. Biochem J. .

Abstract

The exocellular DD-carboxypeptidase-endopeptidase of Streptomyces albus G was purified to protein homogeneity and compared with the exocellular DD-carboxypeptidases-transpeptidases of Streptomyces R61 and Actinomadura R39. The S. albus G enzyme, as it is isolated, occurs in two forms. Enzyme I (30% of the total amount) and enzyme II (70% of the total amount) are identical in all respects, except that, by polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate, enzyme I has an apparent mol. wt. (9000) that is half of that found by molecular-sieve filtration under non-denaturing conditions. Irrespective of the technique used, enzyme II has an apparent mol. wt. of about 18500.

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