Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1980 Mar 25;8(6):1383-90.
doi: 10.1093/nar/8.6.1383.

Methylated bases in mycoplasmal DNA

A Razin, S Razin
Free PMC article
Comparative Study

Methylated bases in mycoplasmal DNA

A Razin et al. Nucleic Acids Res. .
Free PMC article

Abstract

The DNAs of four Mycoplasma and one Acholeplasma species were found to contain methylated bases. All of the five species contained 6-methyladenine (m6Ade), the methylated base characteristic of prokaryotic DNA. The extent of methylation of adenine residues in the mycoplasmal DNA ranged from 0.2% in Mycoplasma capricolum to about 2% in Mycoplasma arginini and Mycoplasma hyorhinis with intermediate methylation values for Mycoplasma orale and Acholeplasma laidlawii DNAs. About 5.8% of the cytosine residues in M. hyorhinis DNA were methylated also. Analysis of cell culture DNA for the presence of m6Ade as a means for detection of contamination by mycoplasmas, and the phylogenetic implications of the finding of methylated bases in mycoplasmal DNAs are discussed.

PubMed Disclaimer

References

    1. Anal Biochem. 1970 Mar;34:188-205 - PubMed
    1. J Mol Biol. 1970 Oct 28;53(2):251-9 - PubMed
    1. J Mol Biol. 1968 Sep 14;36(2):195-208 - PubMed
    1. J Biol Chem. 1977 Aug 10;252(15):5509-13 - PubMed
    1. Microbiol Rev. 1978 Jun;42(2):414-70 - PubMed

Publication types