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Comparative Study
. 1980 May 10;8(9):1999-2008.
doi: 10.1093/nar/8.9.1999.

Variations in mitochondrial DNA organisation between normal and male-sterile cytoplasms of maize

R D Thompson, R J Kemble, R B Flavell
Free PMC article
Comparative Study

Variations in mitochondrial DNA organisation between normal and male-sterile cytoplasms of maize

R D Thompson et al. Nucleic Acids Res. .
Free PMC article

Abstract

Mitochondrial DNA from male-sterile lines of maize carrying S cytoplasm contains two small DNA species which are absent from N (fertile) and other male-sterile cytoplasms. Portions of these species have been purified and amplified by constructing recombinant plasmids in vitro. Probes made with these plasmids have been used to demonstrate; i) a homologous region in the N mitochondrial genome, which may indicate the origin of the S specific DNA species. ii) two other DNA species present in low amounts in S cytoplasm only. iii) the absence of strong homology to the S specific DNA species in mitochondria from C and T male-sterile cytoplasms.

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