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. 1978 Dec 6;69(3):331-8.
doi: 10.1007/BF00332136.

Responses of mammalian metaphase chromosomes to endonuclease digestion

C G Sahasrabuddhe, S Pathak, T C Hsu

Responses of mammalian metaphase chromosomes to endonuclease digestion

C G Sahasrabuddhe et al. Chromosoma. .

Abstract

Digestion of fixed metaphase chromosomes by endonucleases (micrococcal nuclease and DNase II) under optimal digestion conditions followed by Giemsa staining produces sharp banding patterns identical to G-bands. In 3H-thymidine labeled, synchronized metaphase cells of the chinese hamster (CHO line), the band induction is accompanied by the removal of DNA. The single strand specific nuclease S1 and DNase I do not produce such banding patterns.

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