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Comparative Study
. 1980 Nov;33(11):1082-5.
doi: 10.1136/jcp.33.11.1082.

Rubella-specific IgM detection using Sephacryl S-300 gel filtration

P Morgan-Capner, E Davies, J R Pattison
Comparative Study

Rubella-specific IgM detection using Sephacryl S-300 gel filtration

P Morgan-Capner et al. J Clin Pathol. 1980 Nov.

Abstract

Sephacryl S-300 has been compared with Sephadex G-200 in the fractionation of sera for the detection of rubella-specific IgM. No difference in sensitivity was found with sera that had low titres of rubella-specific IgM. Sephadex G-200 was apparently more sensitive with some sera which had high titres of rubella-specific IgM due to the contribution of rubella-specific IgA. Sephacryl S-300 offers considerable advantages in ease and speed of preparation, and as it is a more rigid gel higher flow rates can be obtained. Sera may be fractionated in 2.5 hours on Sephacryl S-300 compared with 7-10 hours on Sephadex G-200.

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References

    1. Med J Aust. 1972 Nov 25;2(22):1244-8 - PubMed
    1. J Clin Pathol. 1975 May;28(5):377-82 - PubMed
    1. J Hyg (Lond). 1975 Aug;75(1):91-8 - PubMed
    1. J Clin Pathol. 1975 Aug;28(8):670-3 - PubMed
    1. J Immunol Methods. 1978;19(2-3):269-77 - PubMed

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