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. 1980 Oct 31;6(3):159-61.
doi: 10.1007/BF00775410.

R-loop hybridization of collagen DNA: separation in Cs2SO4 gradients

J Born, B Wittig, S Wittig, H Tiedemann

R-loop hybridization of collagen DNA: separation in Cs2SO4 gradients

J Born et al. Mol Biol Rep. .

Abstract

High molecular weight poly(A)RNA (26-35 S) from chicken embryo trunks which is enriched for collagen mRNA was iodinated and hybridized to DNA under conditions of R-loop formation. The R-loops were separated in Cs2SO4 gradients from the bulk of DNA yielding double stranded DNA enriched for collagen genes.

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References

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