The lipid composition of light and heavy myelin subfractions isolated from rabbit sciatic nerve

Abstract

Centrifugation of adult rabbit sciatic nerve homogenates on continuous sucrose density gradients yields three distinct maxima at 0.12, 0.33 and 0.57 M sucrose (labeled A, B and C), respectively. Fraction A is absent in homogenates of immature rabbit sciatic nerve and the material isolated from adult animals is found to contain large amounts of triglycerides. It is proposed that fraction A results from the presence of adipose tissue adhering to the adult nerve preparations and should not be regarded as a true myelin subfraction. The concentration of the major membrane lipid classes (phospholipid, cholesterol and galactosyl ceramide) decreases from the light to the heavy side of the gradient. The ethanolamine phosphatide to sphingomyelin ratio increases from 1.06 to 1.42 between fractions B and C, due to an increase in the phosphatidal ethanolamine content at the expense of sphingomyelin.