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. 1980 Sep 11;8(17):3743-55.
doi: 10.1093/nar/8.17.3743.

Nuclease digestion promotes structural rearrangements in H1-depleted chromatin

W O Weischet, K E Van Holde
Free PMC article

Nuclease digestion promotes structural rearrangements in H1-depleted chromatin

W O Weischet et al. Nucleic Acids Res. .
Free PMC article

Abstract

Digestion of H1-depleted chromatin with micrococcal nuclease at an ionic strength of 0.35M gives rise to structural rearrangements indicating nucleosomal sliding. The ionic strength necessary to reveal this effect is significantly lower than that required in the absence of an accompanying digestion. As an explanation, a model is presented in which the progressing terminal degradation of oligomeric nucleosomes is made responsible for promoting structural rearrangements.

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