doi: 10.1093/nar/8.20.4613.
Pyrimidine-specific chemical reactions useful for DNA sequencing
- PMID: 7443522
- PMCID: PMC324374
- DOI: 10.1093/nar/8.20.4613
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Pyrimidine-specific chemical reactions useful for DNA sequencing
Nucleic Acids Res.
.
Free PMC article
Abstract
Potassium permanganate reacts selectively with thymidine residues in DNA (1) while hydroxylamine hydrochloride at pH 6 specifically attacks cytosine (2). We have adopted these reactions for use with the chemical sequencing method developed by Maxam and Gilbert (3).
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