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. 1980 Oct 24;8(20):4613-9.
doi: 10.1093/nar/8.20.4613.

Pyrimidine-specific chemical reactions useful for DNA sequencing

C M Rubin, C W Schmid
Free PMC article

Pyrimidine-specific chemical reactions useful for DNA sequencing

C M Rubin et al. Nucleic Acids Res. .
Free PMC article

Abstract

Potassium permanganate reacts selectively with thymidine residues in DNA (1) while hydroxylamine hydrochloride at pH 6 specifically attacks cytosine (2). We have adopted these reactions for use with the chemical sequencing method developed by Maxam and Gilbert (3).

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References

    1. Nucleic Acids Res. 1978 Feb;5(2):615-22 - PubMed
    1. Nature. 1980 Mar 27;284(5754):372-4 - PubMed
    1. Methods Enzymol. 1980;65(1):499-560 - PubMed
    1. Biochem Biophys Res Commun. 1967 Nov 30;29(4):556-61 - PubMed

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