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. 1980 Aug;23(8):1053-60.

Large-scale purification of prothoracicotropic hormone of the silkworm (Bombyx mori)

H Nagasawa, F Guo, X C Zhong, B Y Xia, Z S Wang, X J Qui, D Y Wei, E I Chen, J Z Wang, A Suzuki, A Isogai, Y Hori, S Tamura, H Ishizaki
  • PMID: 7444426

Large-scale purification of prothoracicotropic hormone of the silkworm (Bombyx mori)

H Nagasawa et al. Sci Sin. 1980 Aug.

Abstract

Prothoracicotropic hormone has been successfully extracted from as many as 2,800,000 silkworm male adult heads and purified partially through an 8-step procedure. Approximately 7 microgram of the crude preparation ("crude PTTH") shows activity in the debrained Samia pupa test. A further 4-step chromatographic purification of the "crude PTTH" derived from 100,000 Bombyx heads yields 3.4 mg of purified preparation ("highly purified PTTH"), of which 7 ng causes adult development in a debrained Samia pupa.

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