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. 1980;46(4):301-12.
doi: 10.1007/BF00421977.

False-negative oxidase reaction as a result of medium acidification

A H Havelaar, C J Hoogendorp, A J Wesdorp, W A Scheffers

False-negative oxidase reaction as a result of medium acidification

A H Havelaar et al. Antonie Van Leeuwenhoek. 1980.

Abstract

Acidification of the culture medium may lead to a false-negative result of the oxidase reaction. Hence, if the medium contained a carbon source or another component that is convertible to acid, a negative oxidase reaction should be considered inconclusive. In order to eliminate false-negative reactions, a reagent freshly adjusted to pH 5-7 might be used; this reagent should be checked on known oxidase-negative strains. However, we recommend that the oxidase test be performed with the normal reagent after (sub)culturing on a non-acidogenic medium.

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