Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1980 Jul;35(1):266-9.
doi: 10.1111/j.1471-4159.1980.tb12515.x.

The use of Sep-Pak C18 cartridges during the isolation of gangliosides

M A Williams, R H McCluer

The use of Sep-Pak C18 cartridges during the isolation of gangliosides

M A Williams et al. J Neurochem. 1980 Jul.

Abstract

The use of Sep-Pak C18 reverse-phase cartridges to adsorb gangliosides from aqueous solutions was studied. When upper phases formed from chloroform-methanol tissue extracts or aqueous salt solutions containing gangliosides are rapidly passed through the cartridges, the lipids are retained and the non-lipid components can be washed through. Gangliosides and other retained lipids can subsequently be eluted with methanol or chloroform-methanol

PubMed Disclaimer

Publication types

LinkOut - more resources