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. 1978 Oct 30;166(2):141-9.
doi: 10.1007/BF00285916.

Electron microscopic analysis of in vitro transcriptional complexes: mapping of promoters of the coliphage T5 genome

D Stüber, H Delius, H Bujard

Electron microscopic analysis of in vitro transcriptional complexes: mapping of promoters of the coliphage T5 genome

D Stüber et al. Mol Gen Genet. .

Abstract

Transcriptional complexes formed in vitro with coliphage T5 DNA as template were analyzed by electron microscopy and the number and location of starting sites utilized by E. coli RNA polymerase were determined. Of the 40 promoters characterized in this way, 6 map in the two terminal "pre-early" regions, 29 in the "early" and 5 in the "late" region. The direction of transcription within the different regions determined in this study agrees with earlier findings derived from RNA synthesized in vivo.

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References

    1. J Mol Biol. 1976 Nov 5;107(3):369-78 - PubMed
    1. Eur J Biochem. 1973 Mar 15;33(3):517-28 - PubMed
    1. CRC Crit Rev Microbiol. 1975 Dec;4(2):101-59 - PubMed
    1. Biochim Biophys Acta. 1971 Sep 30;247(1):181-4 - PubMed
    1. J Mol Biol. 1973 Mar 15;74(4):677-87 - PubMed

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